A CRISPR library points to oil-boosting genes in rapeseed

Rapeseed is one of the world’s most important oil crops, but improving seed oil content and other complex traits still depends on finding genes that can be tested directly in breeding-relevant plants. A new clustered regularly interspaced short palindromic repeats-associated nuclease 9 (CRISPR-Cas9) mutant library built in the elite commercial cultivar Zhongshuang 11 (ZS11) offers such a route. By generating hundreds of edited rapeseed lines, screening visible and seed-quality traits, and vali